human myoblast cell line ab1190 Search Results


human immortalized myoblasts  (PromoCell)
94
PromoCell human immortalized myoblasts
Human Immortalized Myoblasts, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human myoblast cell line ab1190  (PromoCell)
96
PromoCell human myoblast cell line ab1190
( A-B ) Representative immunoblots of immunoprecipitates of CHC22, from control HeLa (A) and differentiated human skeletal muscle (hSKMC) cell line <t>AB1190</t> (B). Samples were immunoblotted for CHC22, SNX5, SNX6, and tubulin. Three lanes comprise: Input (5%), bead-only control (Bead) and CHC22 immunoprecipitate (22 IP). The migration positions of MW markers are indicated at the left in kDa (n = 4-6). ( C ) Schematic illustration of the structure of a single clathrin triskelion comprising three heavy chains, hub region of the protein is indicated by red dotted triangle. ( D ) AlphaFold prediction of the interaction between CHC22 hub and the BAR domain of SNX5. The proximal leg of CHC22 is shown in blue, the trimerization domain (TxD, residues 1520-1640) of CHC22 is shown in magenta, the SNX5 BAR domain is shown in orange. Two angles of view are displayed. ( E ) A representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (His-22) or CHC17 hub (His-17), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 hub (His-22), and His-tagged CHC17 hub (His-17). The position of MW markers is indicated at the left in kDa (n = 3-5). ( F ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (22 Hub) or CHC22 TxD (22 TxD), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 Hub (22 Hub), and His-tagged CHC22 TxD (22 TxD). The position of MW markers is indicated at the left in kDa (n = 3-5). ( G ) Schematic illustration of the SNX1-SNX5 dimer which comprises the ESCPE-1 complex (SNX1 in cyan, SNX5 in orange). ( H ) Overlay of the ESCPE-1 dimer with the Hub region of CHC22 (Proximal legs in blue, TxD region in magenta). ( I ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 TxD (22 TxD) and full-length GST-SNX5 mutants. Samples were immunoblotted for GST and His. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Six lanes in each comprise: bead-only control (GST), wild-type GST-SNX5 (WT), cargo mutant GST-SNX5 (F136D), Phosphomimetic GST-SNX5 (S226A), Phosphomutant GST-SNX5 (S226E) and CHC22 TxD input (2%) (TxD) . The position of MW markers is indicated at the left in kDa (n = 2).
Human Myoblast Cell Line Ab1190, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
human myoblast cell line ab1190 - by Bioz Stars, 2026-03
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anti dnmt1  (Abcam)
96
Abcam anti dnmt1
( A-B ) Representative immunoblots of immunoprecipitates of CHC22, from control HeLa (A) and differentiated human skeletal muscle (hSKMC) cell line <t>AB1190</t> (B). Samples were immunoblotted for CHC22, SNX5, SNX6, and tubulin. Three lanes comprise: Input (5%), bead-only control (Bead) and CHC22 immunoprecipitate (22 IP). The migration positions of MW markers are indicated at the left in kDa (n = 4-6). ( C ) Schematic illustration of the structure of a single clathrin triskelion comprising three heavy chains, hub region of the protein is indicated by red dotted triangle. ( D ) AlphaFold prediction of the interaction between CHC22 hub and the BAR domain of SNX5. The proximal leg of CHC22 is shown in blue, the trimerization domain (TxD, residues 1520-1640) of CHC22 is shown in magenta, the SNX5 BAR domain is shown in orange. Two angles of view are displayed. ( E ) A representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (His-22) or CHC17 hub (His-17), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 hub (His-22), and His-tagged CHC17 hub (His-17). The position of MW markers is indicated at the left in kDa (n = 3-5). ( F ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (22 Hub) or CHC22 TxD (22 TxD), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 Hub (22 Hub), and His-tagged CHC22 TxD (22 TxD). The position of MW markers is indicated at the left in kDa (n = 3-5). ( G ) Schematic illustration of the SNX1-SNX5 dimer which comprises the ESCPE-1 complex (SNX1 in cyan, SNX5 in orange). ( H ) Overlay of the ESCPE-1 dimer with the Hub region of CHC22 (Proximal legs in blue, TxD region in magenta). ( I ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 TxD (22 TxD) and full-length GST-SNX5 mutants. Samples were immunoblotted for GST and His. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Six lanes in each comprise: bead-only control (GST), wild-type GST-SNX5 (WT), cargo mutant GST-SNX5 (F136D), Phosphomimetic GST-SNX5 (S226A), Phosphomutant GST-SNX5 (S226E) and CHC22 TxD input (2%) (TxD) . The position of MW markers is indicated at the left in kDa (n = 2).
Anti Dnmt1, supplied by Abcam, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti dnmt1 - by Bioz Stars, 2026-03
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histone modification antibodies  (Active Motif)
99
Active Motif histone modification antibodies
( A-B ) Representative immunoblots of immunoprecipitates of CHC22, from control HeLa (A) and differentiated human skeletal muscle (hSKMC) cell line <t>AB1190</t> (B). Samples were immunoblotted for CHC22, SNX5, SNX6, and tubulin. Three lanes comprise: Input (5%), bead-only control (Bead) and CHC22 immunoprecipitate (22 IP). The migration positions of MW markers are indicated at the left in kDa (n = 4-6). ( C ) Schematic illustration of the structure of a single clathrin triskelion comprising three heavy chains, hub region of the protein is indicated by red dotted triangle. ( D ) AlphaFold prediction of the interaction between CHC22 hub and the BAR domain of SNX5. The proximal leg of CHC22 is shown in blue, the trimerization domain (TxD, residues 1520-1640) of CHC22 is shown in magenta, the SNX5 BAR domain is shown in orange. Two angles of view are displayed. ( E ) A representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (His-22) or CHC17 hub (His-17), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 hub (His-22), and His-tagged CHC17 hub (His-17). The position of MW markers is indicated at the left in kDa (n = 3-5). ( F ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (22 Hub) or CHC22 TxD (22 TxD), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 Hub (22 Hub), and His-tagged CHC22 TxD (22 TxD). The position of MW markers is indicated at the left in kDa (n = 3-5). ( G ) Schematic illustration of the SNX1-SNX5 dimer which comprises the ESCPE-1 complex (SNX1 in cyan, SNX5 in orange). ( H ) Overlay of the ESCPE-1 dimer with the Hub region of CHC22 (Proximal legs in blue, TxD region in magenta). ( I ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 TxD (22 TxD) and full-length GST-SNX5 mutants. Samples were immunoblotted for GST and His. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Six lanes in each comprise: bead-only control (GST), wild-type GST-SNX5 (WT), cargo mutant GST-SNX5 (F136D), Phosphomimetic GST-SNX5 (S226A), Phosphomutant GST-SNX5 (S226E) and CHC22 TxD input (2%) (TxD) . The position of MW markers is indicated at the left in kDa (n = 2).
Histone Modification Antibodies, supplied by Active Motif, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
histone modification antibodies - by Bioz Stars, 2026-03
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human cystatin c antibody  (R&D Systems)
N/A
The Human Cystatin C Antibody from R D Systems is a mouse monoclonal antibody to Cystatin C This antibody reacts with human The Human Cystatin C Antibody has been validated for the following applications Western
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human tweak r tnfrsf12 antibody  (R&D Systems)
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The Human TWEAK R TNFRSF12 Antibody from R D Systems is a rat monoclonal antibody to TWEAK R TNFRSF12 This antibody reacts with human The Human TWEAK R TNFRSF12 Antibody has been validated for the
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human igg alexa fluor« 700 conjugated antibody  (R&D Systems)
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The Human IgG Alexa Fluor« 700 conjugated Antibody from R D Systems is a mouse monoclonal antibody to IgG This antibody reacts with human The Human IgG Alexa Fluor« 700 conjugated Antibody has been validated
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human dppiv cd26 alexa fluor« 750 conjugated antibody  (R&D Systems)
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The Human DPPIV CD26 Alexa Fluor« 750 conjugated Antibody from R D Systems is a rat monoclonal antibody to DPPIV CD26 This antibody reacts with human The Human DPPIV CD26 Alexa Fluor« 750 conjugated Antibody
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spike rbd antibody (1049349) [allophycocyanin]  (Novus Biologicals)
N/A
The Spike RBD Antibody (1049349) [Allophycocyanin] from Novus is a Spike RBD antibody to Spike RBD. This antibody reacts with SARS-CoV-2. The Spike RBD antibody has been validated for the following applications: Flow Cytometry, Immunocytochemistry.
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igg antibody (97924) [dylight 755]  (Novus Biologicals)
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The IgG Antibody (97924) [DyLight 755] from Novus is a IgG antibody to IgG. This antibody reacts with Human. The IgG antibody has been validated for the following applications: Western Blot, Flow Cytometry, CyTOF-ready.
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igg antibody (97924) [pe/atto594]  (Novus Biologicals)
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The IgG Antibody (97924) [PE/Atto594] from Novus is a IgG antibody to IgG. This antibody reacts with Human. The IgG antibody has been validated for the following applications: Flow Cytometry.
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igg antibody (97924) [pe/cy5.5]  (Novus Biologicals)
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The IgG Antibody (97924) [PE/Cy5.5] from Novus is a IgG antibody to IgG. This antibody reacts with Human. The IgG antibody has been validated for the following applications: Flow Cytometry.
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Image Search Results


( A-B ) Representative immunoblots of immunoprecipitates of CHC22, from control HeLa (A) and differentiated human skeletal muscle (hSKMC) cell line AB1190 (B). Samples were immunoblotted for CHC22, SNX5, SNX6, and tubulin. Three lanes comprise: Input (5%), bead-only control (Bead) and CHC22 immunoprecipitate (22 IP). The migration positions of MW markers are indicated at the left in kDa (n = 4-6). ( C ) Schematic illustration of the structure of a single clathrin triskelion comprising three heavy chains, hub region of the protein is indicated by red dotted triangle. ( D ) AlphaFold prediction of the interaction between CHC22 hub and the BAR domain of SNX5. The proximal leg of CHC22 is shown in blue, the trimerization domain (TxD, residues 1520-1640) of CHC22 is shown in magenta, the SNX5 BAR domain is shown in orange. Two angles of view are displayed. ( E ) A representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (His-22) or CHC17 hub (His-17), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 hub (His-22), and His-tagged CHC17 hub (His-17). The position of MW markers is indicated at the left in kDa (n = 3-5). ( F ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (22 Hub) or CHC22 TxD (22 TxD), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 Hub (22 Hub), and His-tagged CHC22 TxD (22 TxD). The position of MW markers is indicated at the left in kDa (n = 3-5). ( G ) Schematic illustration of the SNX1-SNX5 dimer which comprises the ESCPE-1 complex (SNX1 in cyan, SNX5 in orange). ( H ) Overlay of the ESCPE-1 dimer with the Hub region of CHC22 (Proximal legs in blue, TxD region in magenta). ( I ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 TxD (22 TxD) and full-length GST-SNX5 mutants. Samples were immunoblotted for GST and His. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Six lanes in each comprise: bead-only control (GST), wild-type GST-SNX5 (WT), cargo mutant GST-SNX5 (F136D), Phosphomimetic GST-SNX5 (S226A), Phosphomutant GST-SNX5 (S226E) and CHC22 TxD input (2%) (TxD) . The position of MW markers is indicated at the left in kDa (n = 2).

Journal: bioRxiv

Article Title: CHC22 clathrin membrane recruitment uses SNX5 in bipartite interaction with secretory tether p115

doi: 10.1101/2022.12.21.520923

Figure Lengend Snippet: ( A-B ) Representative immunoblots of immunoprecipitates of CHC22, from control HeLa (A) and differentiated human skeletal muscle (hSKMC) cell line AB1190 (B). Samples were immunoblotted for CHC22, SNX5, SNX6, and tubulin. Three lanes comprise: Input (5%), bead-only control (Bead) and CHC22 immunoprecipitate (22 IP). The migration positions of MW markers are indicated at the left in kDa (n = 4-6). ( C ) Schematic illustration of the structure of a single clathrin triskelion comprising three heavy chains, hub region of the protein is indicated by red dotted triangle. ( D ) AlphaFold prediction of the interaction between CHC22 hub and the BAR domain of SNX5. The proximal leg of CHC22 is shown in blue, the trimerization domain (TxD, residues 1520-1640) of CHC22 is shown in magenta, the SNX5 BAR domain is shown in orange. Two angles of view are displayed. ( E ) A representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (His-22) or CHC17 hub (His-17), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 hub (His-22), and His-tagged CHC17 hub (His-17). The position of MW markers is indicated at the left in kDa (n = 3-5). ( F ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 hub (22 Hub) or CHC22 TxD (22 TxD), and full-length SNX5 (GST-SNX5). Samples were immunoblotted for GST, His and SNX5. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Four lanes in each comprise: Input (2%), bead-only control (Bead), His-tagged CHC22 Hub (22 Hub), and His-tagged CHC22 TxD (22 TxD). The position of MW markers is indicated at the left in kDa (n = 3-5). ( G ) Schematic illustration of the SNX1-SNX5 dimer which comprises the ESCPE-1 complex (SNX1 in cyan, SNX5 in orange). ( H ) Overlay of the ESCPE-1 dimer with the Hub region of CHC22 (Proximal legs in blue, TxD region in magenta). ( I ) Representative immunoblot of in vitro binding assays between purified fragments of: CHC22 TxD (22 TxD) and full-length GST-SNX5 mutants. Samples were immunoblotted for GST and His. Control GST-only lanes are on the left and GST-SNX5 conjugate-containing lanes on the right. Six lanes in each comprise: bead-only control (GST), wild-type GST-SNX5 (WT), cargo mutant GST-SNX5 (F136D), Phosphomimetic GST-SNX5 (S226A), Phosphomutant GST-SNX5 (S226E) and CHC22 TxD input (2%) (TxD) . The position of MW markers is indicated at the left in kDa (n = 2).

Article Snippet: The human myoblast cell line AB1190 was grown in complete Skeletal Muscle Cell Growth Medium (Promocell) with provided supplemental cocktail and 10% FBS (Gibco) to reach a 15% final supplement concentration (volume/volume) ( ).

Techniques: Western Blot, Migration, In Vitro, Binding Assay, Purification, Mutagenesis